“again / re-” in Japanese is 再, read sai, futata (さい, ふたた). It is a Grade 5 Kyōiku kanji, taught in year 5 of Japanese elementary school, and appears in words such as 再生 (regeneration).
What does 再 mean?
もう一度、再び挑戦する。
Same sound, different character — the classic trap. Compare them side by side: